In which colony did the first representative assembly take place?

The first representative assembly in the American colonies was established in Virginia in 1619. This legislative body, known as the House of Burgesses, marked a significant step towards self-governance in the colonies by allowing settlers to elect representatives to make decisions on their behalf. The formation of this assembly laid the groundwork for representative government in other colonies and was a precursor to later democratic practices in the United States. Virginia's early adoption of such a political structure highlights the colony's pivotal role in the evolution of American government.

Other options, while significant in their own rights, either adopted representative government later or implemented different forms of governance. Massachusetts, Connecticut, and Pennsylvania developed their legislative bodies after Virginia, contributing to the broader context of colonial governance but not being the first.