In which colony did the first representative assembly take place?

The first representative assembly in the American colonies took place in Virginia, specifically the House of Burgesses, established in 1619. This event marked a significant development in colonial governance, as it introduced the concept of elected representatives making decisions on behalf of the colonists. The House of Burgesses allowed landowners to elect representatives, thus laying the groundwork for future democratic governance in America.

Virginia's decision to form a representative assembly was a response to the growing need for a structured government that could address the needs and concerns of its settlers. This assembly set a precedent that would influence the formation of political systems in other colonies, but it was in Virginia that the idea of representation took root first, showcasing the colony's early commitment to self-governance and the principles of democracy.

Other options, while they may have had governance structures or local meetings, did not establish the precedent of a representative assembly in the same manner or at such an early date as Virginia did.